Sunday, December 29, 2019

Harry Potter - The Next Chapter - 1202 Words

Harry looked at me. He looked a little shocked, scared, and sad. Understandably. He had just read a letter from his dead mother. Our dead mother. How odd. I didn’t know how I would tell my living parents†¦ The Grangers. I eventually decided that they didn’t need to know. Whether they know they adopted me or they were obliviated and think I am their daughter, no harm would come to them by my not telling them about this. Harry handed my letter to Ron, who read it as well. â€Å"Blimey! So you two, you’re brother and sister? That’s bloody mad!† he exclaimed in his cute, crude, blunt way. â€Å"So, you get why you can’t tell anyone, right? Not even your family?† I asked Ron. â€Å"’Course, Mione, but you won’t even tell the Grangers?† â€Å"Why†¦show more content†¦Ã¢â‚¬Å"I think we should,† I say resolutely. Harry and Ron looked at each other. â€Å"Same here,† said Ron. Harry motioned to Ginny and Luna. â€Å"Hey guys, we have something to show†¦er†¦tell you†¦ just not here. Later. How about the astronomy tower at 9 o’clock? If you see Neville, tell him to come too, cool?† Harry said. Luna, Ron, his sister, and I showed signs of affirmation and Luna and Ginny scattered, probably to their common rooms to get their coats for the match. A few hours later, I was sitting in the Quidditch stands with Ginny, Lavender, and Parvati. Ron was actually playing quite well and had stopped several attempts on the rings. Harry was flying around very high, looking for the snitch and vaguely watching the proceedings with the Quaffle meters below. Every time Ronald made a save, Lavender, sitting to my right screeched in some unearthly pitch and blubbered over how wonderful he was. I wanted to vomit. And no, I wasn’t jealous. He didn’t like her, so there was nothing to be jealous of. Gryffindor and Slytherin were tied at 60-60. Harry seemed to finally see the snitch, and Draco, watching Harry, flew off after him. The two boys went into a steep dive and were moving so fast I felt queasy. Oh, Harry! Be careful!! I could hardly watch. Quidditch scared me. Watching my best friends flying at extremely high speeds, with heavy Bludgers flying around trying to knock them off their thin, little brooms to the hard ground, so far below.Show MoreRelatedHarry Potter : The Book Is Better1304 Words   |  6 PagesIn recent years, two books have come about to be immensely popular on the market. Those books are Twilight and Harry Potter. Harry Potter is about adolescent wizard and his best friends; they attend a school of Witchcraft and Wizardry. The main story is Harry s quest to defeat the evil wizard Lord Voldemort. He wants to control the wizarding world and overpower non-magical people; he wants to get rid of his oppressors which just happen to be, Harry’s paren ts. Twilight is about a girl named IsabellaRead MoreHarry Potter And The Sorcerers Stone Analysis1171 Words   |  5 PagesGasped Harry† (Rowling, 51)- Harry Potter who has been living with the cruel Dursleys, discovers he is a wizard. In   J.K. Rowling’s Harry Potter and the Sorcerer’s Stone, Harry manages his first trip to Diagon Alley. Additionally, Harry Potter and the Prisoner of Azkaban by J.K. Rowling involves Harry Potter utilizing the Marauders Map.   Ã‚  Ã‚  Ã‚  Harry Potter manages a trip to Diagon Alley with Hagrid with the help of his well-known name and curiosity. In chapter 5 â€Å"Diagon Alley†, Harry Potter wakes upRead MoreEssay about The Fame and Fortune of J.K. Rowling1419 Words   |  6 Pagesthe years we have watched J.K. Rowling tower over all other authors in the world. Rowling went through many trials, tribulations and hard ships to be the great woman she is today. Rowling’s life is now in the public eye all because of one boy, Harry Potter. J.K. Rowling once said â€Å"I thought I’d written something that a handful of people might like. So this has been something of a shock.†- J.K. Rowling (Rowling J.K.). Subsequently Rowling’s life will be forever changed. Joanne Kathleen Rowling hasRead MoreWizards and Vampires: Exploring the Phenomenal Popularity of the Harry Potter and Twilight Book Series1536 Words   |  7 Pagesâ€Å"There was a time when no one had heard of Harry Potter,† a title of an article published in the 2007 edition of the Chicago tribune. It attempted to describe the Harry Potter phenomenon. However, if written now it would be â€Å"There was a time when no one had heard of Harry Potter and Edward Cullen† the Twilight series has sparked a phenomenon equal to the Harry Potter series. To know exactly why these fictional books have become a vast phenomenon is impossible but gaining thoughts and slight understandingRead MoreHarry Potter vs. Edward Cullen Essay983 Words   |  4 Pagesno one had heard of Harry Potter,† a title of an article published in the 2007 edition of the Chicago tribune. It attempted to describe the Harry Potter phenomenon. However, if written now it would be â€Å"There was a time when no one had heard of Harry Potter and Edward Cullen† the Twilight series has sparked a phenomenon equal to the Harry Potter series. The debate over which book is better is huge between the clashing fa ns, witches and wizards vs. vampires and werewolves, Harry vs. Edward, MagicRead MoreThe Way Of The World Essay1580 Words   |  7 Pagesbecomes more fragmented in novels. The Following four works; â€Å"Harry Potter and the prisoner of Azkaban†(1999), â€Å"The David Copperfield†(1850), â€Å"The Prime of Miss Jean Brodie†(1961) and â€Å"Dead Poets society†(1989) are defined as the literary genre ‘Bildungsroman’ in different Medias. Each of the works has different analysis of Bildungsroman as the methods of maturity. First of all, Harry Potter and the prisoner of Azkaban is the third chapter of the seven magical series by J.K. Rowling. This is one ofRead MoreHarry Potter Series By J.k. Rowling1398 Words   |  6 Pagesrise to popularity, the widely famous and popular Harry Potter series by J.K. Rowling has always caused conversation. This is because the series of seven novels addresses a variety of social issues. One of these social issues is the idea of the â€Å"Other.† The concept of the â€Å"Other† appears in the Harry Potter universe in several ways, but this essay will focus on the connection between the â€Å"Other† and muggle-borns and their supporters. In Harry Potter and the Deathly Hallows, muggle-born witches andRead MoreHarry Potter and the Half-Blood Prince Reveiw1484 Words   |  6 Pagesâ€Å"When I first saw Harry Potter and the Half-Blood Prince, I exited the theater with an excruciating headache, a lingering sense of emptiness and a very strong desire to punch the film’s cinematographer,† (Ewing) which relates to the movie Harry Potter and the Half-Blood Prince which stars Daniel Radcliffe, Michael Gambon, Jim Broadbent, and directed by David Yates. In the sixth Harry Potter Saga, Harry goes to school, becomes obsessed with a mysterious book which belongs to the Half-Blood PrinceRead MoreThe Pros And Cons Of Harry Potter1487 Words   |  6 PagesSince Harry Potter’s release there has been much conversation surrounding the controversies around it. From being considered occultic and anti-Christian to being thought of as poison to children’s minds, Harry Potter earned a spot on the list of books banned from being taught in public schools. There were many arguments concerning Harry Potter. While being written and published as a young adult book meant for ages 10 and up, many adults did not find it suitable for this age group. Jacqui KomschliesRead MoreJ.k Rowling s The Big Hit Series Of Harry Potter756 Wor ds   |  4 PagesJ.K rowling a british author that wrote the big hit series of harry potter. But she didn t start out famous and her idea for the book wasn t instantly accepted by publishers. Before Harry Potter Rowling was poor Rowling had to go to several different publishers before she found one that would publish her book. Now she became a known author and eventually the first person to become a billionaire from writing books J.K. rowling is a person worthy of praise because she overcame britain adversed to

Saturday, December 21, 2019

Transformation And Electrophoresis Lab Report - 1750 Words

Singee Nguyen Transformation and Electrophoresis Lab Report Purposes Discuss the principles of bacterial transformation. Describe how to prepare competent E. coli cells. Discuss the mechanisms of gene transfer using plasmid vectors. Discuss the transfer of antibiotic resistance genes and tell how to select positively for transformed cells that are antibiotic resistant. Discuss the mechanisms of action for restriction endonucleases. Discuss how a plasmid can be engineered to include a piece of foreign DNA that alters the phenotype of transformed cells. Understand and be able to explain the principles of electrophoresis as they pertain to separating and identifying DNA fragments. Hypothesis If genetic transformation allows the insertion of a gene into an organism to gain the trait that the inserted gene codes for, then when 250  µl of transformation solution(CaCl2) and 2-4 colonies of bacteria, E. coli, are added to two micro test tubes, labelled +pGLO and –pGLO, with a loopful of pGLO DNA solution in the +pGLO tube, and are incubated for 10 minutes, then transferred to a 42 ºC heat bath for exactly 50 seconds, and then incubated in ice again for 2 minutes for a heat shock, and then have 250  µl of LB nutrient broth, and lastly 100  µl of the solutions spread across the surfaces of 4 LB nutrient agar plates, LB/amp, LB/amp/ara, LB/amp, and LB, with +pGLO, +pGLO, –pGLO, and –pGLO used, respectively, the plates with the –pGLO solution will not glow under a UV light after they haveShow MoreRelatedEssay on Pglo Transformation Lab Report1486 Words   |  6 PagesTransformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehowRead MoreAn Understanding Of Bacterial Transformation By Plasmid Dna1874 Words   |  8 Pages Bacterial Transformation Lab Report Elizabeth Amanquah Genetics 311 Monday 2:00-4:50 Jan Trybula Abstract This study was undertaken to develop an understanding of bacterial transformation by plasmid DNA. This study enables one to observe various traits displayed by transformed bacterial cells. Four experiments were conducted that included (1) Bacterial Transformation, (2) Genomic/Plasmid extraction, (3) DNA Electrophoresis, and(4) Plasmid /Oxidation EMSA. In this study, a transformationRead MoreThe Principles Of Bacterial Transformation2210 Words   |  9 PagesPurposes: Discuss the principles of bacterial transformation Describe how to prepare competent E.coli cells Discuss the mechanisms of gene transfer using plasmid vectors Discuss the transfer of antibiotic resistance genes and tell how to select positively for transformed cells that are antibiotic resistance Discuss the mechanisms of action for restriction endonucleases Discuss how a plasmid can be engineered to include a peice of foreign DNA that alters the phenotype of transformed cells UnderstandRead MoreMolecular Genetics 16s Lab Report Essay2106 Words   |  9 PagesMolecular Genetics 16s Lab Report Abstract A highly conserved gene will be used to identify a prokaryotic species isolated from the body. Fundamental lab techniques will be also explored and utilized, such as amplifying using PCR, cloning, and transforming the gene into a host cell. DNA electrophoresis and specific substrate plating will serve as analysis check points. The final product will be sequenced and compared to similar species to observe phylogenetic relationships. IntroRead MoreSalmonella Bacteria For Producing Protein Of Interest And Rate Growth And Protein Concentration After Inoculate The E.coli Culture1137 Words   |  5 PagesGOALS The goals of this lab report are to induce E.coli bacteria for producing protein of interest and determined the rate growth and protein concentration after inoculate the E.coli culture. In addition, protein visualizing and analyzing the success of IPTG induction, protein solubility and affinity chromatography demonstrated using SDS-PAGE. This report will also highlight the methodology of affinity chromatography that used to purify fused protein with poly His tag. A comparison between the advantageRead MoreManipulation And Analysis Of Dna Using Standard Molecular Biology Essay717 Words   |  3 Pagesisolated, with restriction enzymes. Practical 2 (Part B) Transformation of bacterial cells (E. coli) with: a) No DNA b) Plasmid DNA c) digested DNA Practical 3 Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B) Whilst the practical classes deal with different techniques, it is important that you write it up as a single laboratory report. The write-up should contain a general introduction writtenRead MoreThe Mechanisms Linking Ribosomal Translation And Pre Mrna Processing10128 Words   |  41 Pages Investigation of the mechanisms linking ribosomal translation and pre-mRNA processing in yeast Nine-Month Report submitted by Marija Petrić ID: 1454106 Supervisor: Dr Saverio Brogna Contents Contents 1. Introduction 3 1.1. Eukaryotic gene expression 3 1.2. Quality control of eukaryotic gene expression 5 1.3. Nonsense mediated mRNA decay 6 1.4. Factors involved in NMD 7 1.5. NMD models 17 1.6. Ribosome release model 21 1.7. Role of UPF1 in the nucleus 23 2. Objectives 25 3. Materials and methodsRead MoreDna Report4127 Words   |  17 Pages Valerie SelsEmail: saburo_aikini441@yahoo.com | Lab Report DNA: Plasmids and Nucleases 1. Abstract The goal of this practicum was to isolate plasmid DNA from Escherichia coli (E. coli), to identify it, to prove that the plasmid is circular and double-stranded and to give bacterial cells new genetic properties via transformation. An unknown plasmid S was isolated from the bacterial stain Escherichia coli (E. coli). Then its purity

Friday, December 13, 2019

Tidal energy Free Essays

1. Introduction Tidal energy is one of the renewable energies that have a promising hereafter as energy beginnings for the whole universe in general and for some states in peculiar. The purpose of this study is to discourse the current position of tidal energy in footings of engineering, runing rule, environmental effects and its hereafter development. We will write a custom essay sample on Tidal energy or any similar topic only for you Order Now The specific aims are:  · To sketch the current potency of tidal energy throughout the universe  · To discourse the engineerings up to day of the month  · To place the pros and cons of the current engineerings  · To indicate out the environmental impacts of tidal energy and if any accomplishments so far to relieve this consequence  · To discourse the possible hereafter developments 2. Background The Earth and the Moon continuously produce centrifugal forces on one another. This gravitative consequence creates a bump in the oceans confronting to the side of the Moon. A 2nd bump is produced by the centrifugal force due to the rotary motion of the Earth on the opposite side of it. Those two sides of the Earth are the two countries where high tides are produced. The usage of tidal energy has been started since 12th century for grain milling intents in England and France though it has been barely used due to decelerate gait of engineering developments and other inexpensive energy beginning rivals such as fossil fuels. There were no much researches and developments on the engineering and it did n’t acquire adequate attending till recent old ages. But those tidal Millss are the base for the development of modern tidal energy engineerings. 3. Tidal energy Status 3.1. World Tidal Energy Potential The universe ‘s 70 % of its surface covered by H2O has an ability of bring forthing 50000MW tidal current energy yearly ( Atlantis Resource Corporation, 2009 ) . Most of the countries sited in 3.1 are those countries with high potency of tidal energy resources available. But tidal energy is available all over the Earth though the sum of energy to be harnessed is smaller compared to the given 1s. 3.2. Current Tidal Energy Technologies Tidal energy is the energy exploited from the possible and kinetic flow of the tides. There are two presently matured engineerings used to pull out this energy. The possible energy of the tides is exploited utilizing tidal bombardments and the kinetic energy is extracted by the tidal current turbine engineerings ( Fergal O Rourke, 2009 ) . 3.2.1. Tidal Barrages Tidal bombardments are dams constructed at the oral cavity of estuaries to keep up the coming H2O by shuting the piece gates in either side which builds up a possible energy that can be converted in to electricity by utilizing turbines. This has similar rule like the hydro electric power coevals. Advantages  · No demand of fuel  · No waste and nursery gas production  · There is no H2O and air pollution  · Reliable production of electricity  · Predictable and renewable Disadvantage  · Expensive for bombardment edifice  · There is deficit of tidal bombardment sites Policy shapers and companies are loath to pass clip and money in research and development for a wider execution of tidal bombardment engineerings because of their high civil substructure costs and environmental issues. Alternatively, they are concentrating on new engineerings such as tidal current turbines which can minimise the cost of dam buildings due to their simpleness in working rule and suitableness to deploy in different countries of oceans. 3.2.2. Tidal current turbines Tidal current turbines utilises the kinetic energy of H2O currents to revolve the turbines for electricity coevals. The working rule is the same as the windmills that use the air currents to revolve the air current turbines. The transition of kinetic energy in to utile energy is carried out by two widely used turbine systems viz. horizontal and perpendicular axis turbines. Those are the chief competent right now and they are largely under active research stage with a batch of presentations and trials around the universe ( M.J. Khan, G. Bhuyan, M.T. Iqbal, J.E. Quaicoe, 2009 ) . The blades of Horizontal axis tidal turbines rotate about a horizontal axis which is parallel to the flow of H2O as shown in fig 3.3 below. Those constellations and type of blades are non the lone 1s developed so far. Those are merely samples to demo physically how the horizontal axis tidal turbines look like. The blades of Vertical axis tidal turbines rotate about a perpendicular axis which is perpendicular to the way of the flow of H2O as shown in fig 3.4. There are different blade constellations and types under this class though they are non given in this paper. Tidal current turbines have same advantage as the tidal barrages do but relieve the major disadvantages because of their inexpensive building costs and can be deployed in wider countries of the universe. Those are the grounds tidal current turbines are going more attractive and favorable over tidal bombardments and batch of researches and developments are focussed towards those engineerings. 3.3. Environmental impacts Affects the upstream and downstream environment due to the demand of big countries 3.4. Future development 4. Drumhead and decision 5. Mentions Atlantis Resource Corporation. ( 2009 ) . . Retrieved December 27, 2009, from Global Resource: hypertext transfer protocol: //www.atlantisresourcescorporation.com/marine-power/global-resources.html Brooks, R. ( 2008 ) . The World Offshore Renewable Energy Report 2004-2008. DTI. Energy Resources. ( 2009 ) . Retrieved December 05, 2009, from Tidal Power- Energy from the Sea: hypertext transfer protocol: //www.darvill.clara.net/altenerg/tidal.htm Fergal O Rourke, F. B. ( 2009 ) . Tidal energy update. Applied Energy, 398–409. GCK Technology Ltd. ( 2008 ) . Retrieved December 28, 2009, from The Gorlov Helical Turbine: hypertext transfer protocol: //www.gcktechnology.com/GCK/pg2.html M.J. Khan, G. Bhuyan, M.T. Iqbal, J.E. Quaicoe. ( 2009 ) . Hydrokinetic Energy Conversion Systems and Assessment of Horizontal and Vertical Axis Turbines for river and Tidal Applications. Applied Energy, 1823-1835. Peter Clark, Rebecca Klossner, Lauren Kologe. ( 2003 ) . Tidal Energy. How to cite Tidal energy, Essay examples